The correct order of the PCR steps when amplifying DNA in a thermocycler is denaturation, annealing, and extension.
A specific segment of DNA can be quickly produced (amplified) in millions to billions of copies using the polymerase chain reaction (PCR).
PCR is a technique that is based on the three straightforward steps needed for any DNA synthesis reaction:
DNA polymerase, an enzyme, is used in PCR to create numerous copies of specific DNA fragments. This approach, which is significantly more effective than the cloning of expressed genes, enables the production of literally billions of DNA molecules in a matter of hours.
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