Respuesta :
Since the interaction between Arg681 and Cys683 is intra-subunit, the two residues must be located on the same chain.
What is intra-subunit interaction?
The bacterial condensing is an SMC-like protein complex called Mukes. It is most likely that it compacts DNA through a DNA loop-extrusion mechanism that is ATP hydrolysis dependent, as has been shown for the yeast SMC proteins condensing and cohesion. Despite suppressing Muc ATPase activity, Muc also interacts with the Parch subunit of the cellular chromosomal decatenate topoisomerase IV, which is necessary for normal chromosome condensation and segregation in E. coli. In our investigation of the Mukes ATPase function, we used protein-protein crosslinking and site-specific mutagenesis to pinpoint connections between and within subunits.
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