The membrane between the cytoplasm and the mitochondria is impermeable to the passage of electrons through it and as a result of glycolysis the electron that is produced is during glycolysis are transported for the purpose of gain of energy during oxidative phosphorylation.
Malate shunt
In eukaryotes, the mitochondrion's semipermeable inner membrane serves as a conduit for the malate-aspartate shuttle, a biochemical pathway that transports electrons generated during glycolysis across the membrane for oxidative phosphorylation. To produce ATP, these electrons use reduction equivalents to enter the mitochondria's electron transport chain. The shuttle system is necessary because NADH, the principal reducing equivalent of the electron transport chain, cannot get through the mitochondrial inner membrane. Malate transports the reducing equivalents across the membrane to get around this.
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