It might indicate NO fermentation and oxidation of glucose occurred. An oxidative organism breaks down glucose in presence of Oxygen, while a nonoxidative organism can break down glucose in absence of oxygen.
An Oxidation-Fermentation (OF)-glucose test is a microbiological test to determine how an organism is able to break down glucose.
In the first tube, a layer of mineral oil is added in order to reproduce anaerobic conditions, whereas this layer is not added to the second tube in order to reproduce aerobic conditions.
When the non-oil tube turns yellow, while the oil tube remains green, it means that the microorganism is oxidative (i.e., the microorganism only can metabolize glucose in presence of oxygen).
If both tubes remain green, it means that the test is negative and no fermentation/oxidation of glucose occurred.
If both tubes turn yellow, then the microorganism is oxidative and fermentative.
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