4. Arrange Write the following steps in the order in which they occur during glycolysis

A six-carbon molecule is broken down into two three-carbon molecules.

Two NADP molecules are converted into two NADH molecules.

Two phosphate groups from two ATP molecules are joined to glucose.

Two three-carbon molecules are converted into two Folecules of pyruvate as four
molecules of ATP are produced.

Two phosphate groups from two ATP molecules are joined to glucose.
Two NADP molecules are converted into two NADH molecules.
A six-carbon molecule is broken down into two three-carbon molecules.
Two three-carbon molecules are converted into two molecules of pyruvate as four molecules of ATP are produced.

During glycolysis, the glucose molecule is converted to Glucose-6-phosphate as a result of phosphorylation. The phosphate group from ATP is joined to the glucose molecule by an enzyme known as hexokinase.

The glucose-6-phosphate then undergoes isomerization to become fructose-6-phosphate which is then phosphorylated again by the hexokinase enzyme to become fructose-1,6-biphosphate.

An aldolase enzyme converts fructose-1,6-biphosphate to one molecule of glyceraldehyde-3-phosphate and one molecule of dihydroxyacetone phosphate. The latter is then converted to another molecule of glyceraldehyde-3-phosphate.

A dehydrogenase enzyme converts glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate with NADH being generated from the reduction of NAD+.

A kinase enzyme then converts 1,3-bisphosphoglycerate to 3-phosphoglycerate with ATP being generated from ADP. A mutase enzyme quickly converts the 3-phosphoglycerate to 2-phosphoglycerate while an enolase enzyme converts the latter to phosphoenolpyruvate.

Finally, a pyruvate kinase enzyme converts phosphoenolpyruvate to pyruvate with another ATP being generated.

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