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Students have expressed a protein according to the BASIL protocols. They collected a pre-induction sample, a post-induction sample, and a sample following an affinity column purification. The samples were analyzed by SDS-PAGE followed by Coommassie Blue staining. Which lane corresponds to which sample?

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Answer:

The first lane with the protein band is the post-induction sample, i.e., the lane with the sample of interest.

Explanation:

Antibody binding is specific and will only bind to whichever protein it has been produced for particularly, which in this case would be the post induction sample.

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