Which clue indicates that a speciation has occurred between two populations? A. Two individuals within the same population look different from each other. B. A population is separated by a physical barrier such as a river. C. A population has been renamed by scientists. D. Interbreeding between two populations is no longer possible

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I believe that the answer is D. If something has evolved, it can not interbreed with its old species. I think this is right!
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the answer is D!! 
because Speciation is the formation of new and distinct species in the course of evolution. And if the population has already changed to the point that they cant even breed anymore than evolution has occured. In other words, THE ANSWER IS D!
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